addgene pls scel (Addgene inc)
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addgene pls scel
Addgene Pls Scel, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+plasmid/pLS-SceI+(Plasmid+%23137725)/pmc13657374-119-6-6
Average 95 stars, based on 50 article reviews
Addgene Pls Scel, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+plasmid/pLS-SceI+(Plasmid+%23137725)/pmc13657374-119-6-6
Average 95 stars, based on 50 article reviews
addgene pls scel - by Bioz Stars,
2026-10
95/100 stars
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Recombinant:Article Title: Epstein-Barr virus gp350 neutralizing epitopes define distinct sites of vulnerability. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies M13 Bacteriophage Antibody (HRP) Sino Biological Cat#11973-MM05T-H-100 anti-human CD20 Abcam Cat#ab78237; RRID: AB_1640323 72A1 Hoffman et al.20 N/A 1A12 This paper N/A 4A11 This paper N/A 1H5 This paper N/A 2E9 This paper N/A 2G4 Audet et al.37 N/A anti-human IgG HRP antibody Abcam ab97160; RRID: AB_10688252 Bacterial and virus strains EBV CNE2 GFP Zhang et al.38 N/A E.coli: TG1 strain NBbiolab Cat# P008 Helper phage: M13KO7 NEB Cat# N0315S Chemicals, peptides, and recombinant proteins Sodium butyrate Sigma Aldrich Cat# V900464 12-O-tetradecanoylphorbol-13-acetate Beyotime Cat# S1819 BsmBI-v2 NEB Cat# R0739L Busulfan Sigma-Aldrich Cat# 55–98–1 Streptavidin-PE conjugated Bioss Cat# bs-0437P-PE Recombinant EBV gp350 ectodomain This paper N/A Critical commercial assays Ni Sepharose excel Cytiva Cat#17371202 rProtein A Sepharose Fast Flow resin Cytiva Cat#17127906 GoScriptTM Reverse Transcription System Promega Cat# A5001 T7 High Yield RNA Transcription kit Novoprotein Cat# E131 DNA extraction kit TIANGEN Cat#DP304-03 EBER detection kit ZSGB-BIO Cat# ISH-7001–100 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 EZ-LinkTM Sulfo NHS-LC-LC-biotin ThermoFisher Cat#A35358 Blood DNA Extraction Kit TIANGEN Cat #DP304-03 BCA Protein Assay Kit Thermo Fisher Cat#23225 AceQ U+ Probe Master Mix Vazyme Cat#Q113-02 Streptavidin-coated magnetic beads Thermo Fisher Cat#65001 Deposited data CryoEM Maps of gp350–3Fab complex This paper EMDB: EMD-63748 CryoEM Maps of gp350-4A11 complex This paper EMDB: EMD-63745 Atomic Model of gp350–3Fab complex This paper PDB: 9MA7 Atomic Model of gp350-4A11 complex This paper PDB: 9MA0 Experimental models: Cell lines 293F cell BDBIO Cat#C5866 293T cell ATCC Cat#CRL-3216; RRID: CVCL_0063 (Continued on next page) Cell Reports 45, 118072, October 27, 2026 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raji ATCC Cat#CCL-86; RRID: CVCL_0511 Akata iCell Bioscience Cat#iCell-h433: RRID: CVCL_0148 CNE2 FUHENG BIOLOGY Cat#FH0010: RRID: CVCL_6889 BJAB YUBO Biotechnology Cat#YB-H284; RRID: CVCL_5711 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293 ATCC Cat#CRL-1573; RRID: CVCL_0045 Experimental models: Organisms/strains Humanized mouse model BEIJING IDMO Co., Ltd. HuNPI Oligonucleotides BamHI-W primers sense: 5′-GGACCACTGCCCCTGGTATAA-3′ This paper NA BamHI-W primers antisense: 5′-TTTGTGTGGACTCCTGGCG-3′ This paper NA FAM-labeled probe 5′-FAMTCCTGCAGCTATTTCTGGTCGCATCABHQ1-3′ This paper NA Recombinant DNA Pcomb3XSS Andris-Widhopf et al.39 Article Title: Purinergic receptor activation rectifies autism-associated endothelial dysfunction. Article Snippet: Report Purinergic receptor activation rectifies autismassociated endothelial dysfunction Highlights • 16p11.2-deficient brain endothelial cells display a bioenergetic failure • ATP signaling rescues the function of 16p11.2-deficient brain endothelial cells • P2Y2 receptor activation restores the function of 16p11.2deficient endothelial cells • P2Y2 receptor activation improves blood flow and behavior in 16p11.2-deficient mice Authors Julie Ouellette, Sareen Warsi, Phinea Romero, ..., Fabrice Dabertrand, Armen Saghatelyan, Baptiste Lacoste Correspondence blacoste@uottawa.ca In brief Ouellette et al. report that a 16p11.2 deletion induces a bioenergetic failure in brain endothelial cells.. Activation of purinergic signaling via P2Y2 receptors restored endothelial function and improved behaviors in mice carrying a 16p11.2 deletion.. Targeting endothelial metabolism and purinergic signaling may thus represent a promising therapeutic avenue for autism. Article Title: Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1. Article Snippet: Report Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1 Highlights • Shear stress induces transient donut-shaped mitochondria in kidney epithelial cells • Physiological flow transiently stabilizes ER-mitochondria contact sites • Shear stress promotes ER-lipid droplet-mitochondria triple contact-site formation • VMP1 is required for fatty-acid transfer from lipid droplets to mitochondria Authors Maria Laura Rojas, Maribel Lara Corona, Carla Alemany, ..., Ida Chiara Guerrera, Aurore Claude-Taupin, Etienne Morel Correspondence aurore.claude-taupin@inserm.fr (A.C.-T.), etienne.morel@inserm.fr (E.M.) In brief Rojas et al. show that physiological shear stress triggers rapid mitochondrial remodeling and transient stabilization of ER-mitochondria contact sites in kidney epithelial cells.. These contacts recruit lipid droplets, forming triple organelle junctions that facilitate local fatty-acid transfer to mitochondria, a process requiring the ER scramblase VMP1.. Rojas et al, 2026, Current Biology 36, 1–11 October 19, 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies Plasmid Preparation:Article Title: Epstein-Barr virus gp350 neutralizing epitopes define distinct sites of vulnerability. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies M13 Bacteriophage Antibody (HRP) Sino Biological Cat#11973-MM05T-H-100 anti-human CD20 Abcam Cat#ab78237; RRID: AB_1640323 72A1 Hoffman et al.20 N/A 1A12 This paper N/A 4A11 This paper N/A 1H5 This paper N/A 2E9 This paper N/A 2G4 Audet et al.37 N/A anti-human IgG HRP antibody Abcam ab97160; RRID: AB_10688252 Bacterial and virus strains EBV CNE2 GFP Zhang et al.38 N/A E.coli: TG1 strain NBbiolab Cat# P008 Helper phage: M13KO7 NEB Cat# N0315S Chemicals, peptides, and recombinant proteins Sodium butyrate Sigma Aldrich Cat# V900464 12-O-tetradecanoylphorbol-13-acetate Beyotime Cat# S1819 BsmBI-v2 NEB Cat# R0739L Busulfan Sigma-Aldrich Cat# 55–98–1 Streptavidin-PE conjugated Bioss Cat# bs-0437P-PE Recombinant EBV gp350 ectodomain This paper N/A Critical commercial assays Ni Sepharose excel Cytiva Cat#17371202 rProtein A Sepharose Fast Flow resin Cytiva Cat#17127906 GoScriptTM Reverse Transcription System Promega Cat# A5001 T7 High Yield RNA Transcription kit Novoprotein Cat# E131 DNA extraction kit TIANGEN Cat#DP304-03 EBER detection kit ZSGB-BIO Cat# ISH-7001–100 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 EZ-LinkTM Sulfo NHS-LC-LC-biotin ThermoFisher Cat#A35358 Blood DNA Extraction Kit TIANGEN Cat #DP304-03 BCA Protein Assay Kit Thermo Fisher Cat#23225 AceQ U+ Probe Master Mix Vazyme Cat#Q113-02 Streptavidin-coated magnetic beads Thermo Fisher Cat#65001 Deposited data CryoEM Maps of gp350–3Fab complex This paper EMDB: EMD-63748 CryoEM Maps of gp350-4A11 complex This paper EMDB: EMD-63745 Atomic Model of gp350–3Fab complex This paper PDB: 9MA7 Atomic Model of gp350-4A11 complex This paper PDB: 9MA0 Experimental models: Cell lines 293F cell BDBIO Cat#C5866 293T cell ATCC Cat#CRL-3216; RRID: CVCL_0063 (Continued on next page) Cell Reports 45, 118072, October 27, 2026 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raji ATCC Cat#CCL-86; RRID: CVCL_0511 Akata iCell Bioscience Cat#iCell-h433: RRID: CVCL_0148 CNE2 FUHENG BIOLOGY Cat#FH0010: RRID: CVCL_6889 BJAB YUBO Biotechnology Cat#YB-H284; RRID: CVCL_5711 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293 ATCC Cat#CRL-1573; RRID: CVCL_0045 Experimental models: Organisms/strains Humanized mouse model BEIJING IDMO Co., Ltd. HuNPI Oligonucleotides BamHI-W primers sense: 5′-GGACCACTGCCCCTGGTATAA-3′ This paper NA BamHI-W primers antisense: 5′-TTTGTGTGGACTCCTGGCG-3′ This paper NA FAM-labeled probe 5′-FAMTCCTGCAGCTATTTCTGGTCGCATCABHQ1-3′ This paper NA Recombinant DNA Pcomb3XSS Andris-Widhopf et al.39 Article Title: Purinergic receptor activation rectifies autism-associated endothelial dysfunction. Article Snippet: Report Purinergic receptor activation rectifies autismassociated endothelial dysfunction Highlights • 16p11.2-deficient brain endothelial cells display a bioenergetic failure • ATP signaling rescues the function of 16p11.2-deficient brain endothelial cells • P2Y2 receptor activation restores the function of 16p11.2deficient endothelial cells • P2Y2 receptor activation improves blood flow and behavior in 16p11.2-deficient mice Authors Julie Ouellette, Sareen Warsi, Phinea Romero, ..., Fabrice Dabertrand, Armen Saghatelyan, Baptiste Lacoste Correspondence blacoste@uottawa.ca In brief Ouellette et al. report that a 16p11.2 deletion induces a bioenergetic failure in brain endothelial cells.. Activation of purinergic signaling via P2Y2 receptors restored endothelial function and improved behaviors in mice carrying a 16p11.2 deletion.. Targeting endothelial metabolism and purinergic signaling may thus represent a promising therapeutic avenue for autism. Article Title: Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1. Article Snippet: Report Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1 Highlights • Shear stress induces transient donut-shaped mitochondria in kidney epithelial cells • Physiological flow transiently stabilizes ER-mitochondria contact sites • Shear stress promotes ER-lipid droplet-mitochondria triple contact-site formation • VMP1 is required for fatty-acid transfer from lipid droplets to mitochondria Authors Maria Laura Rojas, Maribel Lara Corona, Carla Alemany, ..., Ida Chiara Guerrera, Aurore Claude-Taupin, Etienne Morel Correspondence aurore.claude-taupin@inserm.fr (A.C.-T.), etienne.morel@inserm.fr (E.M.) In brief Rojas et al. show that physiological shear stress triggers rapid mitochondrial remodeling and transient stabilization of ER-mitochondria contact sites in kidney epithelial cells.. These contacts recruit lipid droplets, forming triple organelle junctions that facilitate local fatty-acid transfer to mitochondria, a process requiring the ER scramblase VMP1.. Rojas et al, 2026, Current Biology 36, 1–11 October 19, 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies Article Title: Directing GABAergic neuronal identity from human iPSCs through developmental patterning. Article Snippet: .. FUW-M2rtTA Hockemeyer et al. (2008) Software:Article Title: Purinergic receptor activation rectifies autism-associated endothelial dysfunction. Article Snippet: Report Purinergic receptor activation rectifies autismassociated endothelial dysfunction Highlights • 16p11.2-deficient brain endothelial cells display a bioenergetic failure • ATP signaling rescues the function of 16p11.2-deficient brain endothelial cells • P2Y2 receptor activation restores the function of 16p11.2deficient endothelial cells • P2Y2 receptor activation improves blood flow and behavior in 16p11.2-deficient mice Authors Julie Ouellette, Sareen Warsi, Phinea Romero, ..., Fabrice Dabertrand, Armen Saghatelyan, Baptiste Lacoste Correspondence blacoste@uottawa.ca In brief Ouellette et al. report that a 16p11.2 deletion induces a bioenergetic failure in brain endothelial cells.. Activation of purinergic signaling via P2Y2 receptors restored endothelial function and improved behaviors in mice carrying a 16p11.2 deletion.. Targeting endothelial metabolism and purinergic signaling may thus represent a promising therapeutic avenue for autism. Magnetic Cell Separation:Article Title: Purinergic receptor activation rectifies autism-associated endothelial dysfunction. Article Snippet: Report Purinergic receptor activation rectifies autismassociated endothelial dysfunction Highlights • 16p11.2-deficient brain endothelial cells display a bioenergetic failure • ATP signaling rescues the function of 16p11.2-deficient brain endothelial cells • P2Y2 receptor activation restores the function of 16p11.2deficient endothelial cells • P2Y2 receptor activation improves blood flow and behavior in 16p11.2-deficient mice Authors Julie Ouellette, Sareen Warsi, Phinea Romero, ..., Fabrice Dabertrand, Armen Saghatelyan, Baptiste Lacoste Correspondence blacoste@uottawa.ca In brief Ouellette et al. report that a 16p11.2 deletion induces a bioenergetic failure in brain endothelial cells.. Activation of purinergic signaling via P2Y2 receptors restored endothelial function and improved behaviors in mice carrying a 16p11.2 deletion.. Targeting endothelial metabolism and purinergic signaling may thus represent a promising therapeutic avenue for autism. Microscopy:Article Title: Purinergic receptor activation rectifies autism-associated endothelial dysfunction. Article Snippet: Report Purinergic receptor activation rectifies autismassociated endothelial dysfunction Highlights • 16p11.2-deficient brain endothelial cells display a bioenergetic failure • ATP signaling rescues the function of 16p11.2-deficient brain endothelial cells • P2Y2 receptor activation restores the function of 16p11.2deficient endothelial cells • P2Y2 receptor activation improves blood flow and behavior in 16p11.2-deficient mice Authors Julie Ouellette, Sareen Warsi, Phinea Romero, ..., Fabrice Dabertrand, Armen Saghatelyan, Baptiste Lacoste Correspondence blacoste@uottawa.ca In brief Ouellette et al. report that a 16p11.2 deletion induces a bioenergetic failure in brain endothelial cells.. Activation of purinergic signaling via P2Y2 receptors restored endothelial function and improved behaviors in mice carrying a 16p11.2 deletion.. Targeting endothelial metabolism and purinergic signaling may thus represent a promising therapeutic avenue for autism. Negative Control:Article Title: Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1. Article Snippet: Report Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1 Highlights • Shear stress induces transient donut-shaped mitochondria in kidney epithelial cells • Physiological flow transiently stabilizes ER-mitochondria contact sites • Shear stress promotes ER-lipid droplet-mitochondria triple contact-site formation • VMP1 is required for fatty-acid transfer from lipid droplets to mitochondria Authors Maria Laura Rojas, Maribel Lara Corona, Carla Alemany, ..., Ida Chiara Guerrera, Aurore Claude-Taupin, Etienne Morel Correspondence aurore.claude-taupin@inserm.fr (A.C.-T.), etienne.morel@inserm.fr (E.M.) In brief Rojas et al. show that physiological shear stress triggers rapid mitochondrial remodeling and transient stabilization of ER-mitochondria contact sites in kidney epithelial cells.. These contacts recruit lipid droplets, forming triple organelle junctions that facilitate local fatty-acid transfer to mitochondria, a process requiring the ER scramblase VMP1.. Rojas et al, 2026, Current Biology 36, 1–11 October 19, 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies |